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Campylobacter

Clinical Features :

  • known to cause abortion in domestic animals such as cattle, sheep, and swine
  • sign and symptoms include :
    • diarrhea
    • abdominal cramps
    • anorexia
    • fever
  • C. jejuni is the most common cause of gastroenteritis worldwide
  • C. fetus ssp. fetus is isolated from blood cultures and rarely associated with gastroenteritis illness

General Characteristics :

  • gram negative bacilli with spirals and seagull wings

Culture Media :

  • CAMPY blood agar – use an enriched selective agar ,CAMPY-BAP, to isolate; contains a Brucella agar base with sheep blood and antimicrobials
  • Skirrow
  • Butzler

Environmental Requirements :

  • microaerophilic AND capnophilic environment with gas mixture of approximately 85% N and 5% to 10% O2, 10 % CO2
  • incubation is at 42°C – optimal growth temperature for organisms and other normal flora is inhibited at this temperature

Laboratory Diagnosis :

  • culture media
    • colonies will appear moist, runny, and spreading
  • C. jejuni is hippurate positive
  • gram stain morphology is very important to identify these organisms
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